melatonin receptor mtnr1b specific antagonist 4 p pdot (MedChemExpress)
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melatonin receptor mtnr1b specific antagonist 4 p pdot
Melatonin Receptor Mtnr1b Specific Antagonist 4 P Pdot, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/melatonin+receptor+antagonist+4+p+pdot/4-P-PDOT/pm38960012-56-7-20
Average 94 stars, based on 24 article reviews
Melatonin Receptor Mtnr1b Specific Antagonist 4 P Pdot, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/melatonin+receptor+antagonist+4+p+pdot/4-P-PDOT/pm38960012-56-7-20
Average 94 stars, based on 24 article reviews
melatonin receptor mtnr1b specific antagonist 4 p pdot - by Bioz Stars,
2026-10
94/100 stars
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1) Product Images from "Mtnr1b deletion disrupts placental angiogenesis through the VEGF signaling pathway leading to fetal growth restriction."
Article Title: Mtnr1b deletion disrupts placental angiogenesis through the VEGF signaling pathway leading to fetal growth restriction.
Journal: Pharmacological research
doi: 10.1016/j.phrs.2024.107290
Figure Legend Snippet: Fig. 1. Effects of Mtnr1b knockout in mice on placental functions. (a) Mtnr1b knockout murine construction strategy. (b) PCR results of Mtnr1b knockout at the genomic level. (c) The mRNA expression levels in placental tissues (n = 4). (d) The average litter size (n = 4). (e) Photograph of feta size at day 18 of gestation. (f) The average of fetal weight (n = 33). (g) The average of placental weight (n = 33). (h) The average of placental diameter (n = 33). (i) The average of placental efficiency (n = 33). Data are presented as means ± SEM. * p < 0.05. *** p < 0.001.
Techniques Used: Knock-Out, Expressing
Figure Legend Snippet: Fig. 2. Effects of Mtnr1b knockout in mice on placental apoptosis and antioxidant enzymes. (a) Placental tissue’s TUNEL staining. Scale Bar = 100 μm. (b) The average of fluorescence intensity (apoptotic staining) was statistically analyzed with Image J software (n = 3). (c, d) The expression levels of apoptosis related proteins Cleaved-caspase3 and Bcl-2 in placental tissues (n = 4). (e, f) The expression levels of antioxidant enzymes of Cat and HO-1 in placenta tissues (n = 4). Data are presented as means ± SEM. * p < 0.05. ** p < 0.01.
Techniques Used: Knock-Out, TUNEL Assay, Staining, Fluorescence, Software, Expressing
Figure Legend Snippet: Fig. 4. Effects of Mtnr1b knockout in mice on placental angiogenesis. (a) The mRNA levels of angiogenesis related factors (n = 4). (b) The mRNA levels of VEGF receptors (n = 4). (c-h) The protein expression levels of VEGFR2, p-AKT and eNOS3 in placenta tissues (n = 4). Data are presented as means ± SEM. * p < 0.05. ** p < 0.01.
Techniques Used: Knock-Out, Expressing
Figure Legend Snippet: Fig. 7. Effects of melatonergic system on STAT3/VEGFR2 pathway. (a) The mRNA expression levels of VEGFR2 upon STAT3 activation (n = 3). (b, c) Western blotting was used to detect the expression levels of VEGFR2 and p-STAT3 after STAT3 phosphorylation activation, (n = 3). (d) The mRNA expression levels of VEGFR2 upon inhibition of STAT3 activation (n = 3). (e, f) Western blotting was used to detect the expression levels of VEGFR2 and p-STAT3 after inhibition of STAT3 activation, (n = 3). (g, h) The protein level of VEGFR2 was detected by supplementing STAT3 after MTNR1B inhibition, (n = 3). (i, j) The expression level of p- STAT3 in mouse placenta was detected by Western blotting (n = 4). (k, l) Western blotting was used to detect the expression level of p-STAT3 in porcine placenta (n = 3). Data are presented as means ± SEM. * means p < 0.05. *** means p < 0.001.
Techniques Used: Expressing, Activation Assay, Western Blot, Phospho-proteomics, Inhibition
Related Articles
Incubation:Article Title: Melatonin Alleviates Lipopolysaccharide-Induced Abnormal Pregnancy through MTNR1B Regulation of m6A. Article Snippet: Cells were cultured in DMEMB/F12 (Gibco, Grand Island, NE, USA) containing 10% FBS (Gibco, Grand Island, NE, USA). .. Once cells were attached, HESCs were starved for 12 h in DMEM/F12, cells were treated with 1 μM Melatonin and 50 μg/mL LPS for 48 h. For experiments involving inhibitors, cells were incubated with inhibitors for 48 h. The following specific inhibitors were used: Article Title: Melatonin alleviates LPS-induced abnormal pregnancy through MTNR1B regulation of m6A Article Snippet: .. Once cells were attached, HESCs were starved for 12 h in DMEM/F12, cells were treated with 1 μM Melatonin and 50 μg/mL LPS for 48 h. For experiments involving inhibitors, cells were incubated with inhibitor for 48 h. The following specific inhibitors were used: Article Title: Melatonin Alleviates Lipopolysaccharide-Induced Abnormal Pregnancy through MTNR1B Regulation of m6A Article Snippet: Cells were cultured in DMEMB/F12 (Gibco, Grand Island, NE, USA) containing 10% FBS (Gibco, Grand Island, NE, USA). .. Once cells were attached, HESCs were starved for 12 h in DMEM/F12, cells were treated with 1 μM Melatonin and 50 μg/mL LPS for 48 h. For experiments involving inhibitors, cells were incubated with inhibitors for 48 h. The following specific inhibitors were used: |
